The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric area under curve method for estimation of tinidazole. This area under curve of UV spectrum between 301 to 321 nm was validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 5-30 μg/ml. The value of correlation coefficient was 0.997. Satisfactory values of percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (101.32% to 107.32%) showed accuracy of the method. LOD and LOQ were calculated as 0.6763 μg/ml and 2.049 μg/ml, respectively. The developed method can be used for routine estimation of tinidazole in bulk and tablet dosage forms.
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